Infertility issues not only arise due to problems with women but also due to problems with men. For accurate analysis, Akruti IVF Centre provides advanced andrology facilities, the details of which have been mentioned below.
Advanced Andrology Facilities
Semen analysis is the complete analysis of the semen sample right from it’s volume, appearance, odour, pH, and consistency. Morphology of the sperms is also examined with the help of a pre-stained morphology slide to know the number of sperms with normal forms and the sperms with head, neck or tail defects.
Sperm concentration is counted with the help of Makler Chamber. Ideally sperm concentration should be a minimum of 15 million/ml. If the count is less than this then it is termed as Oligozoospermia. If no sperms are observed in the sample, it is termed as Azoospermia. If sperms are seen in the pellet after centrifugation, then it is termed as Cryptozoospermia. If motility of the sperms is less than 40%, it is termed as Asthenozoospermia. In terms of morphology, if less than 4% of the sperms are with normal forms, it is termed as Teratozoospermia.
DNA fragmentation index
Advanced semen analysis includes calculation of the DNA fragmentation index of the sperms. DNA fragmentation test is carried out with the aid of a DNA fragmentation kit which includes reagents along with hydrophilic agarose coated slide. Through this genetic material of the sperms in a semen sample can be examined and in case of any abnormality, appropriate medications are prescribed by the clinician to reduce the DNA fragmentation of the sperms.
A semen analysis report is given to the patient based on these findings. Our clinician then discusses this report with the patient and gives appropriate medical advice before taking further step.
Semen processing methods
The two most widely used semen processing techniques are Swim Up and Density Gradient.
Swim up method
After the first spin with buffer media, the pellet is layered with either buffer media or culture media. After about 30 mins, the upper layer with good quality sperms is used for further procedures.
Density Gradient method
Two gradients, usually 40% and 80% are used and the sample is centrifuged with them. The second spin is given with sperm wash media and then the pellet is resuspended in buffer media. Also, density gradient is followed by swim up method for better results.
MACS is one of the advanced techniques to retrieve healthy sperms which we use at Akruti IVF Centre. It is a semen processing technique which differentiates healthy sperms from the ones which might undergo apoptosis. This helps in achieving better results in terms of fertilisation rate, cleavage rate, implantation rate and pregnancy rate.
Advanced sperm retrieval techniques such as TESA, TESE, PESA and MESA are opted for the patients with azoospermia. All these processes are carried out with utmost precision by our clinician who is well versed in these techniques.
Testicular Sperm Aspiration (TESA):- It includes insertion of a needle in the testis and aspirating the tissue with negative pressure. The aspirated tissue is then processed by the embryologists in the IVF lab, sperms are extracted and then used for ICSI.
Testicular sperm extraction (TESE):- It is sperm extraction by surgical biopsy of the testis.
Percutaneous epididymal sperm aspiration (PESA):- It includes insertion of a small needle into the epididymis through the scrotal skin and fluid is aspirated. The embryologists then aspirates the sperms from the fluid for ICSI.
Microsurgical Epididymal Sperm Aspiration (MESA):- This involves dissection of the epididymis under the microscope and incision of a single tubule. Fluid is aspirated and sperms are retrieved from the fluid. The selected sperms are then used for ICSI.